Journal: Plant and Soil

Article Title: Arabinose and protocatechuate catabolism genes are important for growth of Rhizobium leguminosarum biovar viciae in the pea rhizosphere

doi: 10.1007/s11104-015-2389-5

Figure Lengend Snippet: R.l. viciae mutants with decreased rhizosphere growth based on microarray analysis of signature tags

Article Snippet: Arrays were hybridized using an Advalytix Array Booster DNA Microarray Incubator (Olympus America Inc., Concord, MA, USA).

Techniques: Microarray

Journal: Plant and Soil

Article Title: Arabinose and protocatechuate catabolism genes are important for growth of Rhizobium leguminosarum biovar viciae in the pea rhizosphere

doi: 10.1007/s11104-015-2389-5

Figure Lengend Snippet: Tests of increased growth of a control strain relative to primary mutants decreased for rhizosphere competitiveness. Mutants identified from microarray experiments as being likely to be defective for rhizoshere competitiveness were mixed with strain RU3940 (which is equally competitive with WT) in a ratio of about 10:1 (except for X365 which was mixed at a ratio of about 100:1) and inoculated into the rhizospheres of one-week-old pea seedlings. After 1 week of growth bacteria were isolated from the rhizospheres. Also shown (control) is a strain which carries mini Tn5 but showed no defect for rhizosphere growth. The black and open bars respectively show the % of strain RU3940 in the initial inoculum and in the rhizosphere as determined by bacterial counts. The data are averages (± SE) and all comparisons (except the control) were significantly different ( p = <0.05) based on Tukey’s HSD Test

Article Snippet: Arrays were hybridized using an Advalytix Array Booster DNA Microarray Incubator (Olympus America Inc., Concord, MA, USA).

Techniques: Microarray, Isolation